hdac3 fluorogenic assay kit  (BPS Bioscience)

Journal: Journal of Neuroinflammation

Article Title: Sodium phenylbutyrate inhibits Schwann cell inflammation via HDAC and NFκB to promote axonal regeneration and remyelination

doi: 10.1186/s12974-021-02273-1

Figure Lengend Snippet: Inflammatory response and the modulation of HDAC expression by LPS in RT4 SCs. A Secretion of TNFα after LPS induction for 24 h were measured by ELISA. B Representative western blot data of changes in the expression of TNFα, c-PARP, and TLR4 expression in a dose-dependent manner, following LPS induction for 24 h. C Expression profile of HDAC1, 2, 3, and 4 after LPS induction for 24 h. D LPS-induced increases of HDAC3 activity were confirmed by HDAC3 activities assay. n = 4. Significance was assessed by one-way ANOVA. The data are presented as the mean ± SEM. * p < 0.05 versus 0 μg/ml LPS. LPS lipopolysaccharide, SCs Schwann cells, TNFα tumor necrosis factor α, c-PARP cleaved poly (ADP) ribose polymerase, HDAC histone deacetylase, TLR4 toll-like receptor 4, ANOVA analysis of variance, SEM standard error of the mean

Article Snippet: The fresh nuclear fractions were also used for measuring the HDAC3 activity using the fluorogenic HDAC3 assay kit (50073, BPS Bioscience, USA).

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Western Blot, Activity Assay, Histone Deacetylase Assay

Journal: Journal of Neuroinflammation

Article Title: Sodium phenylbutyrate inhibits Schwann cell inflammation via HDAC and NFκB to promote axonal regeneration and remyelination

doi: 10.1186/s12974-021-02273-1

Figure Lengend Snippet: PBA reverses LPS-induced inflammatory effects in RT4 SCs. A Western blot analysis shows the decreased expression levels of TNFα, c-PARP, and TLR4 following LPS and PBA co-treatment for 24 h. B LPS-induced secretion of TNFα was inhibited by PBA treatments. C Western blot depicting the changes in HDAC3 expression levels but not in the levels of the other HDACs after 24 h of treatment with LPS and PBA. D HDAC3 activity induced by 24 h of treatment with LPS was also suppressed after PBA treatment. n = 4. Significance was assessed by one-way ANOVA. Data are presented as the mean ± SEM. *p < 0.05 versus no LPS and no PBA. # p < 0.05 versus only LPS. LPS lipopolysaccharide, PBA sodium phenylbutyrate, SCs Schwann cells, TNFα tumor necrosis factor α, c-PARP cleaved poly (ADP) ribose polymerase, TLR4 toll-like receptor 4, HDAC histone deacetylase, ANOVA analysis of variance, SEM standard error of the mean

Article Snippet: The fresh nuclear fractions were also used for measuring the HDAC3 activity using the fluorogenic HDAC3 assay kit (50073, BPS Bioscience, USA).

Techniques: Western Blot, Expressing, Activity Assay, Histone Deacetylase Assay